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Coding
N291D

Part:BBa_K590023:Design

Designed by: Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu   Group: iGEM11_Washington   (2011-09-15)


Kumamolisin-As_N291D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
    Illegal NgoMIV site found at 720
    Illegal NgoMIV site found at 1248
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 772
    Illegal AgeI site found at 1348
    Illegal AgeI site found at 1588
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutation at residue 291 from Asparagine to Aspartate.


Source

In-House Mutant

References